The Relation between Serum Pentraxin 3 Concentrations on Day of Embryo Transfer and Prediction of Pregnancy in Intracytoplasmic Sperm Injection (ICSI) Cycles

Background: Prediction of clinical pregnancy is rapidly becoming an important objective in assisted reproduction technology (ART); on the other hand, most patients are successfully fertilized and get embryos transferred (ET), but not all of transferred embryos actually implant and develop into viable pregnancies.   
Aim of the Work: The aim of the present study was to as-sess the relationship between serum Pentraxin 3 concen-trations on day of embryo transfer and prediction of preg-nancy in intracytoplasmic sperm injection (ICSI) cycles.    
Patients and Methods: This was a prospective observa-tional cohort study, was conducted at Assisted Reproduc-tive Technology Unit (ART Unit) at Ain Shams Univer-sity Maternity Hospitals on 30 Women who underwent intracytoplasmic sperm injection (ICSI). The study start-ed on February 2023 and ended on March 2024. 
Results: Median age of the study population is 30 years, No statistically significant difference (p value >0.05) between pregnant & non pregnant groups as regards de-mographic data, obstetric history, biochemical assay, en-dometrial thickness, days of stimulation, number of go-nadotropins ampoules and oocyte quality. No statistically significant difference between pregnant & non-pregnant groups as regards S.Pentraxin3(p > 0.05) and has poor predictive value Using ROC curve (AUC =. 609, 95% CI =. 414 to. 781, p =. 350). Best cutoff criterion is serum pentraxin 3 > 3.1 ng/ml (sensitivity [95% CI] = 85.7% [42.1% to 99.6%], specificity = [95% CI] = 47.8% (26.8% to 69.4%) Using ROC curve for prediction of biochem-ical pregnancy using E2, E2 has good predictive value (AUC =. 817, 95% CI =. 634 to. 933, p <.001). Best cut-off criterion is E2 ≤41.0 pg/ml (sensitivity [95% CI] = 100% [59.0% - 100.0%], specificity = [95% CI] = 60.9% [38.5% - 80.3%]. Based on these results PTX3 doesn’t seem to be correlated with subsequent pregnancy in ICSI cycles.
Conclusion: The measurement of PTX3 on day of em-bryo transfer is not predictive of subsequent pregnancy as there was no statistically significant difference between pregnant & non pregnant groups as regards PTX3 and there is only weak negative cor-relation between serum pentraxin 3 and BMI.